Improved bacterial affinity and reactive oxygen species generation enhances antibacterial inactivation in wastewater by graphene oxide-wrapped nanospheres developed by scientists at Rice University and Tongji University, Shanghai. Antibiotic resistance genes released by inactivated antibiotic resistant bacteria in the vicinity of photocatalytic sites on the spheres facilitates their degradation. (Graphic courtesy of Alvarez Research Group/Rice University)
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Treatment + Get AlertsThink of the new strategy developed at Rice University as “wrap, trap and zap.”
The labs of Rice environmental scientist Pedro Alvarez and Yalei Zhang — a professor of environmental engineering at Tongji University, Shanghai — recently introduced microspheres wrapped in graphene oxide in the Elsevier journal Water Research.
Alvarez and his partners in the Rice-based Nanosystems Engineering Research Center for Nanotechnology-Enabled Water Treatment (NEWT) have worked toward quenching antibiotic-resistant superbugs since first finding them in wastewater treatment plants in 2013.
“Superbugs are known to breed in wastewater treatment plants and release extracellular antibiotic resistance genes (ARGs) when they are killed as the effluent is disinfected,” Alvarez says. “These ARGs are then discharged and may transform indigenous bacteria in the receiving environment, which become resistome reservoirs.
“Our innovation would minimize the discharge of extracellular ARGs, and thus mitigate dissemination of antibiotic resistance from wastewater treatment plants,” he says.
The Rice lab showed its spheres — cores of bismuth, oxygen and carbon wrapped with nitrogen-doped graphene oxide — inactivated multidrug-resistant E. coli bacteria and degraded plasmid-encoded antibiotic-resistant genes in secondary wastewater effluent.
The graphene-wrapped spheres kill these bacteria in effluent by producing three times the amount of reactive oxygen species (ROS) as compared to the spheres alone.
A scanning electron microscope image shows a graphene oxide shell around the layered nanoplates that make up the core of a particle that traps and zaps antibiotic-resistant bacteria and the resistance genes they release. The wrapped spheres developed at Rice and Tongji universities proved three times better able to disinfect secondary effluent from wastewater plants than the spheres without the nitrogen-doped graphene oxide. (Image courtesy of Deyi Li/Tongji University)
The spheres themselves are photocatalysts that produce ROS when exposed to light. Lab tests showed that wrapping the spheres minimized the ability of ROS scavengers to curtail their ability to disinfect the solution.
How it works
The researchers say nitrogen-doping the shells increases their ability to capture bacteria, giving the catalytic spheres more time to kill them. The enhanced particles then immediately capture and degrade the resistant genes released by the dead bacteria before they contaminate the effluent.
“Wrapping improved bacterial affinity for the microspheres through enhanced hydrophobic interaction between the bacterial surface and the shell,” says co-lead author Pingfeng Yu, a postdoctoral research associate at Rice’s Brown School of Engineering. “This mitigated ROS dilution and scavenging by background constituents and facilitated immediate capture and degradation of the released ARGs.”
Because the wrapped spheres are large enough to be filtered out of the disinfected effluent, they can be reused, Yu says. Tests show the photocatalytic activity of the spheres is relatively stable, with no significant decrease in activity after 10 cycles. That is significantly better than the cycle lifetime of the same spheres minus the wrap.
Deyi Li of Tongji University, Shanghai, is co-lead author of the paper. Co-authors are Xuefei Zhou and Zhang of Tongji and Jae-Hong Kim, the Henry P. Becton Sr. Professor and Chair of Chemical and Environmental Engineering at Yale University. Alvarez is the George R. Brown Professor of Civil and Environmental Engineering, a professor of chemistry, of materials science and nanoengineering and of chemical and biomoleculary engineering and director of NEWT.
The National Science Foundation, the National Natural Science Foundation of China and the National Key R&D Program of China supported the research.
